DIAGNOSTIC ASSAY FOR HELICOBACTER-HEPATICUS BASED ON NUCLEOTIDE-SEQUENCE OF ITS 16S RIBOSOMAL-RNA GENE

被引：46

作者：

BATTLES, JK ^{[1
]}

WILLIAMSON, JC ^{[1
]}

PIKE, KM ^{[1
]}

GORELICK, PL ^{[1
]}

WARD, JM ^{[1
]}

GONDA, MA ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,LAB ANIM SCI PROGRAM,FREDERICK,MD 21702

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 05期

关键词：

D O I：

10.1128/JCM.33.5.1344-1347.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.

引用

页码：1344 / 1347

页数：4