A COMPARISON OF HETEROTOPIC AND ORTHOTOPIC INTESTINAL TRANSPLANTATION IN RATS

被引:38
作者
GRANT, D
ZHONG, R
HURLBUT, D
GARCIA, B
CHEN, HF
LAMONT, D
WANG, PZ
STILLER, C
DUFF, J
机构
[1] UNIV WESTERN ONTARIO, LONDON N6A 3K7, ONTARIO, CANADA
[2] UNIV WESTERN ONTARIO HOSP, JOHN P ROBARTS RES INST, DEPT MED, LONDON N6A 5A5, ONTARIO, CANADA
[3] UNIV WESTERN ONTARIO HOSP, JOHN P ROBERTS RES INST, DEPT PATHOL, LONDON N6A 5A5, ONTARIO, CANADA
关键词
D O I
10.1097/00007890-199105000-00003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Two surgical techniques are commonly used for small intestinal transplantation: heterotopic (accessory) intestinal grafting (HIT), where the small bowel is initially defunctioned with restoration of intestinal continuity at a later date, and orthotopic (in continuity) intestinal grafting (OIT), where the small bowel is immediately anastomosed to the native intestine. The present experiments were undertaken to compare the advantages and disadvantages of these two surgical models. Graft barrier function (intestinal permeability), intestinal histology, and graft survival were evaluated after heterotopic and orthotopic intestinal transplantation in the following groups of rats: group 1: isografts, group 2: untreated allografts, group 3: low-dose cyclosporine-treated allografts (subcutaneous CsA 2 mg/kg/day), and group 4: high-dose CsA-treated allografts (subcutaneous CsA 4 mg/kg/day). Intestinal permeability was consistently higher after HIT than OIT in all of the groups (ANOVA; P < 0.01). Histological evidence of rejection appeared earlier after HIT than OIT (HIT 5th postoperative day (POD); OIT 7th POD; P < 0.05). The mean survival of untreated allografts was longer after HIT than OIT (HIT 15.7 +/- 6 days, OIT 9.2 +/- 1 days, P < 0.05). The rats treated with low-dose CsA after OIT lost weight and died of rejection after a mean survival time of 17.7 +/- 2 days, while the rats treated with low-dose CsA after HIT remained well until sacrifice on POD 28 (P < 0.01). The rats with isografts and rats with allografts treated with high-dose CsA remained well after HIT and OIT until sacrifice on the 28th POD. These data suggest that nutrients and other factors in the succus entericus may improve gut barrier function and delay the onset of rejection after OIT. However, rejection of the orthotopic intestinal graft is usually fatal, while rejection of the heterotopic graft is often surprisingly well tolerated. These factors must be taken into consideration when choosing a surgical technique for intestinal transplantation in humans.
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页码:948 / 954
页数:7
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