DETECTION OF ENDOTHELIN-1 MESSENGER-RNA BY RT-PCR IN ISOLATED RAT RENAL TUBULES

被引：34

作者：

UCHIDA, S

TAKEMOTO, F

OGATA, E

KUROKAWA, K

机构：

[1] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 4,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 1,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)92356-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5′ and 3′ primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues. © 1992.

引用

页码：108 / 113

页数：6

共 17 条

[11] CDNA CLONING, SEQUENCE-ANALYSIS AND TISSUE DISTRIBUTION OF RAT PREPROENDOTHELIN-1 MESSENGER-RNA [J].