GLYCATION-INDUCED CROSS-LINKING OF CALF LENS CRYSTALLINS

被引：24

作者：

VANBOEKEL, MAM

HOENDERS, HJ

机构：

[1] Department of Biochemistry, University of Nijmegen, 6525 EP Nijmegen

来源：

EXPERIMENTAL EYE RESEARCH | 1991年 / 53卷 / 01期

关键词：

GLYCATION; HIGH-MOLECULAR-WEIGHT PROTEIN; CALF LENS; CRYSTALLINS; CROSS-LINKING;

D O I：

10.1016/0014-4835(91)90149-9

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

To investigate the possible role of glycation in the onset of diabetic cataract we used calf lens crystallins as a model. After incubation with reducing sugars, the proteins were investigated by high-pressure gel permeation chromatography, SDS-PAGE and analytical ultracentrifugation. Glucose-6-phosphate incubation resulted in an increase in mean molecular weight of all crystallin fractions and the occurrence of high-molecular weight material, partly formed by disulphide bonds. The glycated crystallins showed a decrease of tryptophan fluorescence and an increase of a specific non-tryptophan fluorescence. This fluorescence was, however, not exclusively associated with the high molecular weight protein, but was present in all protein fractions. © 1991.

引用

页码：89 / 94

页数：6

共 31 条

[1] ROLE OF GLYCOSYLATION IN PROTEIN DISULFIDE FORMATION AND CATARACTOGENESIS [J].