MAPPING OF THE IMMUNOREACTIVE DOMAINS OF A SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED SM-D AUTOANTIGEN

被引：24

作者：

ROKEACH, LA ^{[1
]}

JANNATIPOUR, M ^{[1
]}

HASELBY, JA ^{[1
]}

HOCH, SO ^{[1
]}

机构：

[1] AGOURON INST, 505 COAST BLVD S, LA JOLLA, CA 92037 USA

来源：

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1992年 / 65卷 / 03期

关键词：

D O I：

10.1016/0090-1229(92)90163-I

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The Sm-D(D1) small nuclear ribonucleoprotein (snRNP) polypeptide is a major target of autoantibodies diagnostic for systemic lupus erythematosus. The cDNA encoding the protein from Raji cells was expressed in Escherichia coli as a fusion protein with anthranilate synthase (TrpE-Sm-D). When tested by protein blot, the recombinant polypeptide was strongly immunoreactive under defined blotting conditions, which appear to facilitate the refolding of the polypeptide into a native conformation. Multiple transplational fusions between the trpE gene and fragments encompassing the length of the Sm-D coding sequence were constructed for epitope mapping. The results describe two general patterns of anti-Sm reactivity: (i) antibodies that recognize only the full-length antigen and are presumably directed against discontinuous epitopes, and (ii) antibodies that recognize the carboxy terminus of the antigen which enbodies an extended/charged structure. © 1992.

引用

页码：315 / 324

页数：10

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