SYNTHESIS OF SIMIAN-VIRUS-40 C-FAMILY CATENATED DIMERS IN-VIVO IN THE PRESENCE OF ICRF-193

被引：20

作者：

ISHIMI, Y ^{[1
]}

ISHIDA, R ^{[1
]}

ANDOH, T ^{[1
]}

机构：

[1] AICHI CANC CTR,RES INST,BIOCHEM LAB,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1995年 / 247卷 / 05期

关键词：

SV40; TERMINATION OF DNA REPLICATION; CATENATED DIMER; ICRF-193; TOPOISOMERASE II;

D O I：

10.1006/jmbi.1995.0183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of ICRF-193, a non-cleavable, complex-stabilizing type of topoisomerase II inhibitor, on SV40 DNA replication in vivo was examined. As analyzed by one and two-dimensional gel electrophoresis, C-family catenated dimers, each composed of two intertwined, covalently closed SV40 DNAs, were mainly synthesized in the presence of the drug. On removal of the drug these C-family dimers were segregated into monomers. These results indicate that topoisomerase II is required for the segregation of replicated daughter molecules, but it is not absolutely required for the replication of DNA molecules up to the C-family dimers.

引用

页码：835 / 839

页数：5