DISTINCTIVE UPTAKE OF NEUTRAL RED BY MITOTIC CANCER-CELLS

被引：17

作者：

SIT, KH ^{[1
]}

BAY, BH ^{[1
]}

WONG, KP ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE,FAC MED,DEPT BIOCHEM,SINGAPORE 0511,SINGAPORE

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1992年 / 67卷 / 04期

关键词：

A431; MALIGNANT MELANOMA; ORAL CARCINOMA; HEPATOMA; CHANG LIVER CELLS; EPIDERMAL GROWTH FACTOR; NUCLEAR INFLUX;

D O I：

10.3109/10520299209110066

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Neutral red stains both normal and cancer mitotic cells, but uptake by living mitotic cancer cells is distinctly higher than in normal cells. This new approach to cancer cell identification is demonstrated in 4 established tumorigenic cancer cell lines: human skin epidermoid carcinoma A431, mouse Cloudman malignant melanoma, human oral epidermoid carcinoma and rat hepatoma. Human Chang liver cells served as normal controls. With epidermal growth factor (EGF) prepulse, neutral red uptake is dramatically enhanced. The possibility of a causal relationship with M-phase specific phosphorylation is discussed.

引用

页码：196 / 201

页数：6

共 11 条

[1] Oncogenes and anti-oncogenes [J].

Cooper, J. A. .

CURRENT OPINION IN CELL BIOLOGY, 1990, 2 (02) :285-295

[2] HUMAN CDC2 PROTEIN-KINASE IS A MAJOR CELL-CYCLE REGULATED TYROSINE KINASE SUBSTRATE [J].

DRAETTA, G ;

PIWNICAWORMS, H ;

MORRISON, D ;

DRUKER, B ;

ROBERTS, T ;

BEACH, D .

NATURE, 1988, 336 (6201) :738-744

[3]

GILL GN, 1988, QUANT BIOL L, V3, P467

[4]

GOLDBERG AM, 1989, SCI AM, V261, P16

[5] TYROSINE PHOSPHORYLATION OF THE FISSION YEAST CDC2+ PROTEIN-KINASE REGULATES ENTRY INTO MITOSIS [J].

GOULD, KL ;

NURSE, P .

NATURE, 1989, 342 (6245) :39-45

[6] THE CELL-CYCLE REGULATED PROLIFERATING CELL NUCLEAR ANTIGEN IS REQUIRED FOR SV40-DNA REPLICATION INVITRO [J].

PRELICH, G ;

KOSTURA, M ;

MARSHAK, DR ;

MATHEWS, MB ;

STILLMAN, B .

NATURE, 1987, 326 (6112) :471-475

[7]

REYNOLDS JEF, 1982, MARTINDALE EXTRA PHA, P1732

[8] ANTIPORT-MEDIATED CELL RETRACTION - VIABLE ROUNDING AND DISTINCTIVE ENDOCYTOSIS [J].

SIT, KH ;

BAY, BH ;

WONG, KP .

TISSUE & CELL, 1990, 22 (06) :785-802

[9]

SIT KH, 1989, TISSUE CELL, V21, P388

[10]

WINDHOLZ M, 1983, MERCK INDEX, P930

← 1 2 →