NONISOTOPIC RECEPTOR-BINDING ASSAY FOR BENZODIAZEPINE RECEPTORS UTILIZING A FLUOROPHORE LABELED LIGAND

被引：17

作者：

TAKEUCHI, T ^{[1
]}

RECHNITZ, GA ^{[1
]}

机构：

[1] UNIV HAWAII,DEPT CHEM,HAWAII BIOSENSOR LAB,HONOLULU,HI 96822

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90226-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nonisotopic receptor-binding assay method provides a new approach for the study of receptor-ligand interactions and a possible receptor assay for benzodiazepine drugs. The proposed method is based upon the use of fluorescence-labeled drugs and a chromatographic system which accepts samples without deproteinization. The effectiveness of the technique is illustrated in a study of benzodiazepine receptor-drug-binding interactions. © 1991.

引用

页码：250 / 255

页数：6

共 28 条

[21] NEUROTRANSMITTER RECEPTORS IN BRAIN - BIOCHEMICAL IDENTIFICATION [J].

SNYDER, SH ;

BENNETT, JP .

ANNUAL REVIEW OF PHYSIOLOGY, 1976, 38 :153-175

[22] BENZODIAZEPINE BINDING IN HUMAN-BRAIN - CHARACTERIZATION USING [H-3]FLUNITRAZEPAM [J].

SPETH, RC ;

WASTEK, GJ ;

JOHNSON, PC ;

YAMAMURA, HI .

LIFE SCIENCES, 1978, 22 (10) :859-866

[23]

Squires R., 1988, GABA BENZODIAZEPINE

[24] BENZODIAZEPINE RECEPTORS IN RAT-BRAIN [J].

SQUIRES, RF ;

BRAESTRUP, C .

NATURE, 1977, 266 (5604) :732-734

[25]

TUNE L, 1988, ANAL PSYCHIATRIC DRU, P219

[26]

VELAZQUEZ JL, 1989, J NEUROSCI, V9, P2163

[27] DIRECT INJECTION OF PLASMA INTO COLUMN LIQUID-CHROMATOGRAPHIC SYSTEMS [J].

WESTERLUND, D .

CHROMATOGRAPHIA, 1987, 24 :155-164

[28]

Yamamura H.L., 1985, NEUROTRANSMITTER REC

← 1 2 3 →