EFFECTS OF ACUTE EXPOSURE TO FORMALDEHYDE ON SURFACE-MORPHOLOGY OF NASAL EPITHELIA IN RATS

This study investigated the distribution of epithelial cells over the turbinates in the rat nasal cavity and their injury following exposure to formaldehyde. Rats were exposed to either purified air or 10 ppm formaldehyde for a period of 4 h. The noses removed from these rats were decalcified, sectioned midsagittally along the septum to expose the internal turbinates, and processed for examination by scanning electron microscopy. The distribution of the various cell types present was documented in the control rats. Observations obtained from the formaldehyde-exposed rats were compared to those in rats exposed to purified air. Changes were seen in the various regions of the turbinates in the form of ciliary destruction and cell separation (especially in the naso- and moxilloturbinates), cellular swelling (throughout the turbinates), mucus release by the goblet cells (in the nasoturbinate), and in some cases pores on the cell surface or between adjacent cells (evident in the middle meatus). The data from this study indicate that the degree of deleterious effects of formaldehyde on the nasal epithelia of rats is dependent upon cell type and location. Histological studies supported the scanning electron microscopic observations by demonstrating increased goblet cell volume, cell sloughing, and damaged cilia.