CLONING, EXPRESSION AND CHARACTERIZATION OF HORSE L-FERRITIN IN ESCHERICHIA-COLI

被引：42

作者：

TAKEDA, S

OHTA, M

EBINA, S

NAGAYAMA, K

机构：

[1] Protein Array Project, ERATO, JRDC, Tsukuba

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 02期

关键词：

FERRITIN; RECOMBINANT DNA; AMINO ACID SEQUENCE; (HORSE);

D O I：

10.1016/0167-4781(93)90121-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Horse L-ferritin cDNA was cloned from horse liver, and the base sequence was determined. The L-ferritin was expressed using pTZ18U encoding lac promoter, and found to possess an additional 8-amino acid sequence at the N-terminus as compared with commercially obtained horse spleen (natural) ferritin. It was determined that there was Pro at position 94 in both the recombinant and natural L-ferritin, although it was previously reported that Leu was in this position in the natural species. Transmission electron microscopy showed that this recombinant ferritin formed a 24-mer shell.

引用

页码：218 / 220

页数：3

共 7 条

[1] STRUCTURE, FUNCTION, AND EVOLUTION OF FERRITINS [J].