THE ROLE OF PROTEIN-KINASE-C IN THE INDUCTION OF VCAM-1 EXPRESSION ON HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS

被引：60

作者：

DEISHER, TA ^{[1
]}

HADDIX, TL ^{[1
]}

MONTGOMERY, KF ^{[1
]}

POHLMAN, TH ^{[1
]}

KAUSHANSKY, K ^{[1
]}

HARLAN, JM ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT SURG,SEATTLE,WA 98195

来源：

FEBS LETTERS | 1993年 / 331卷 / 03期

关键词：

PROTEIN KINASE-C; VASCULAR CELL ADHESION MOLECULE-1; TUMOR NECROSIS FACTOR-ALPHA; INTERLEUKIN-1-BETA; LIPOPOLYSACCHARIDE; HUMAN UMBILICAL VEIN ENDOTHELIAL CELL;

D O I：

10.1016/0014-5793(93)80354-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of protein kinase C (PKC) in interleukin-1beta-(Il-1beta)-, tumor necrosis factor-alpha- (TNF-alpha)-, and lipopolysaccharide- (LPS)-induced vascular cell adhesion molecule-1 (VCAM-1) expression on human umbilical vein endothelial cells (HUVEC) was studied. PKC inhibition or downregulation diminished VCAM-1 mRNA accumulation and protein expression. Interleukin-1beta, TNF-alpha, and LPS induce nuclear factor (NF)-kappaB-like binding activity, which precedes VCAM-1 transcription. PKC inhibition did not prevent NF-kappaB-like binding activity, indicating that this is PKC-independent, and NF-kappaB-like binding activity is insufficient for transcription of VCAM-1.

引用

页码：285 / 290

页数：6

共 22 条

[1] A BLOCKING MONOCLONAL-ANTIBODY TO ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULE-1 (ELAM1)
BENJAMIN, C
DOUGAS, I
CHIROSSO, G
LUHOWSKYJ, S
ROSA, M
NEWMAN, B
OSBORN, L
VASSALLO, C
HESSION, C
GOELZ, S
MCCARTHY, K
LOBB, R
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 171 (01) : 348 - 353
[2] CARLOS TM, 1990, BLOOD, V76, P965
[3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[4] A PROTEIN-KINASE-C AGONIST, SELECTIVE FOR THE BETA-I ISOZYME, INDUCES E-SELECTIN AND VCAM-1 EXPRESSION ON HUVEC BUT DOES NOT TRANSLOCATE PKC
DEISHER, TA
SATO, TT
POHLMAN, TH
HARLAN, JM
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 193 (03) : 1283 - 1290
[5] ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - DIRECT EXPRESSION CLONING AND FUNCTIONAL INTERACTIONS
HESSION, C
OSBORN, L
GOFF, D
CHIROSSO, G
VASSALLO, C
PASEK, M
PITTACK, C
TIZARD, R
GOELZ, S
MCCARTHY, K
HOPPLE, S
LOBB, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) : 1673 - 1677
[6] IADEMARCO MF, 1992, J BIOL CHEM, V267, P16323
[7] CALPHOSTIN C (UCN-1028C), A NOVEL MICROBIAL COMPOUND, IS A HIGHLY POTENT AND SPECIFIC INHIBITOR OF PROTEIN KINASE-C
KOBAYASHI, E
NAKANO, H
MORIMOTO, M
TAMAOKI, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (02) : 548 - 553
[8] MODULATION OF ENDOTHELIAL-CELL EXPRESSION OF INTERCELLULAR-ADHESION MOLECULE-1 BY PROTEIN KINASE-C ACTIVATION
LANE, TA
LAMKIN, GE
WANCEWICZ, E
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (03) : 945 - 952
[9] PROTEIN-KINASE-C INHIBITORS BLOCK THE ENHANCED EXPRESSION OF INTERCELLULAR-ADHESION MOLECULE-1 ON ENDOTHELIAL-CELLS ACTIVATED BY INTERLEUKIN-1, LIPOPOLYSACCHARIDE AND TUMOR-NECROSIS-FACTOR
LANE, TA
LAMKIN, GE
WANCEWICZ, EV
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 172 (03) : 1273 - 1281
[10] MAGNUSON DK, 1989, SURGERY, V106, P216

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