A RAPID METHOD OF EVALUATING PERMEABILIZING ACTIVITY AGAINST PSEUDOMONAS-AERUGINOSA

被引：16

作者：

AYRES, H ^{[1
]}

FURR, JR ^{[1
]}

RUSSELL, AD ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,WELSH SCH PHARM,POB 13,CARDIFF CF1 3XF,WALES

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 17卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb00381.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid (< 30 min) spectrophotometric method has been used to assess the ability of chemical agents to permeabilize the outer membrane of Pseudomonas aeruginosa. When permeabilized, the cells are rendered readily sensitive to lysozyme, an enzyme that is normally excluded from the peptidoglycan of this organism. In addition to ethylenediamine tetraacetic acid, substances showing permeabilizing activity were citric, isocitric, malic and gluconic acids (but not gluconic acid lactone) and sodium polyphosphate.

引用

页码：149 / 151

页数：3

共 8 条

[1]

BROWN MRW, 1975, RESISTANCE PSEUDOMON, P71

[2] DAMAGE OF THE OUTER-MEMBRANE OF ENTERIC GRAM-NEGATIVE BACTERIA BY LACTOFERRIN AND TRANSFERRIN [J].

ELLISON, RT ;

GIEHL, TJ ;

LAFORCE, FM .

INFECTION AND IMMUNITY, 1988, 56 (11) :2774-2781

[3]

HAQUE SMH, 1974, ANTIMICROBIAL AGENTS, V6, P200

[4]

NIKAIDO H, 1985, MICROBIOL REV, V49, P1

[5] LYSIS OF GRAM-NEGATIVE ORGANISMS AND THE ROLE OF VERSENE [J].

REPASKE, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1958, 30 (02) :225-232

[6] MECHANISMS OF BACTERIAL-RESISTANCE TO NON-ANTIBIOTICS - FOOD-ADDITIVES AND FOOD AND PHARMACEUTICAL PRESERVATIVES [J].

RUSSELL, AD .

JOURNAL OF APPLIED BACTERIOLOGY, 1991, 71 (03) :191-201

[7]

RUSSELL AD, 1990, UNDERSTANDING ANTIBA, P174

[8] POLYCATIONS SENSITIZE ENTERIC BACTERIA TO ANTIBIOTICS [J].

VAARA, M ;

VAARA, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1983, 24 (01) :107-113

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