LASER-INDUCED FLUORESCENCE DETECTION OF RHODAMINE-6G AT 6 X 10-15 M

被引：15

作者：

HAHN, JH ^{[1
]}

SOPER, SA ^{[1
]}

NUTTER, HL ^{[1
]}

MARTIN, JC ^{[1
]}

JETT, JH ^{[1
]}

KELLER, RA ^{[1
]}

机构：

[1] UNIV CALIF LOS ALAMOS SCI LAB,CTR HUMAN GENOME STUDIES,LOS ALAMOS,NM 87545

来源：

APPLIED SPECTROSCOPY | 1991年 / 45卷 / 05期

关键词：

FLUORESCENCE; LASER; HIGH SENSITIVITY;

D O I：

10.1366/0003702914336453

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We have demonstrated sensitive fluorescence detection of rhodamine-6G in aqueous solutions down to approximately 10(-15) M with a detection limit of 6 x 10(-15) M. The working curve was observed to be linear over four orders of magnitude and could be extended to higher concentrations. Single molecules of rhodamine-6G had a transit time of 1.8 ms through the 11 pL probe volume. At 2 x 10(-14) M, the probability of a molecule being in the 11 pL probe volume is approximately 0.1. Analysis of the data indicates that the signal-to-noise for the detection of a single molecule of rhodamine-6G is approximately 1.

引用

页码：743 / 746

页数：4

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