A DOMINANT NEGATIVE MUTATION OF THE ALPHA-RETINOIC ACID RECEPTOR GENE IN A RETINOIC ACID-NONRESPONSIVE EMBRYONAL CARCINOMA CELL

被引：152

作者：

PRATT, MAC ^{[1
]}

KRALOVA, J ^{[1
]}

MCBURNEY, MW ^{[1
]}

机构：

[1] UNIV OTTAWA,DEPT MED,451 SMYTH RD,OTTAWA K1H 8M5,ONTARIO,CANADA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 12期

关键词：

D O I：

10.1128/MCB.10.12.6445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pluripotential embryonal carcinoma cells such as those of the P19 line differentiate when exposed to retinoic acid (RA). The RAC65 cell line is a mutant clone of P19 cells selected to be RA nonresponsive. RAC65 cells carry a rearrangement affecting one of the genes encoding a nuclear retinoic acid receptor (RARα). The mutant gene encodes a protein, RARα′, that has lost its 70 C-terminal amino acids, thus truncating the RA-binding domain. The RARα′ was found to be a dominant represser of transcription from an RA-responsive target gene; however, expression of RARaα′ was insufficient to confer RA nonresponsiveness, suggesting that RAC65 cells carry an additional mutation(s) affecting RA-induced genes.

引用

页码：6445 / 6453

页数：9

共 72 条

[1]

Adra C.N., Boer P.H., McBurney M.W., Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter, Gene, 60, pp. 65-74, (1987)

[2]

Auffray C., Rougeon F., Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA, Eur. J. Biochem., 107, pp. 303-314, (1980)

[3]

Beato M., Gene regulation by steroid hormones, Cell, 56, pp. 335-344, (1989)

[4]

Benbrook D., Lernhardt E., Pfahl M., A new retinoic acid receptor identified from a hepatocellular carcinoma, Nature (London), 333, pp. 669-672, (1988)

[5]

Bennett K.L., Hill R.E., Pietras D.F., Woodworth-Gutai M., Kane-Hass C., Houston J.M., Heath J.K., Hastie N.D., Most highly repeated dispersed DNA families in the mouse genome, Mol. Cell. Biol., 4, pp. 1561-1571, (1984)

[6]

Berg R.W., McBurney M.W., Cell density and cell cycle effects on retinoic acid induced embryonal carcinoma cell differentiation, Dev. Biol., 138, pp. 123-135, (1990)

[7]

Bladon T.S., Fregean C.J., McBurney M.W., Synthesis and processing of small B2 transcripts in mouse embryonal carcinoma cells, Mol. Cell. Biol., 10, pp. 4058-4067, (1990)

[8]

Blin N., Stafford D.W., A general method for isolation of high molecular weight DNA from eukaryotes, Nucleic Acids Res., 3, pp. 2303-2308, (1976)

[9]

Boer P.H., Potter M., Adra C.N., Jardine K., Mullhofer G., McBurney M.W., Polymorphisms in the coding and noncoding regions of murine pgk-1 alleles, Biochem. Genet., 28, pp. 299-308, (1990)

[10]

Brand N.J., Petkovich M., Krust A., Chambon P., De The H., Marchio A., Tiollais P., Dejean A., Identification of a second human retinoic acid receptor, Nature (London), 332, pp. 850-853, (1988)

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