A DOMINANT NEGATIVE MUTATION OF THE ALPHA-RETINOIC ACID RECEPTOR GENE IN A RETINOIC ACID-NONRESPONSIVE EMBRYONAL CARCINOMA CELL

被引：152

作者：

PRATT, MAC ^{[1
]}

KRALOVA, J ^{[1
]}

MCBURNEY, MW ^{[1
]}

机构：

[1] UNIV OTTAWA,DEPT MED,451 SMYTH RD,OTTAWA K1H 8M5,ONTARIO,CANADA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 12期

关键词：

D O I：

10.1128/MCB.10.12.6445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pluripotential embryonal carcinoma cells such as those of the P19 line differentiate when exposed to retinoic acid (RA). The RAC65 cell line is a mutant clone of P19 cells selected to be RA nonresponsive. RAC65 cells carry a rearrangement affecting one of the genes encoding a nuclear retinoic acid receptor (RARα). The mutant gene encodes a protein, RARα′, that has lost its 70 C-terminal amino acids, thus truncating the RA-binding domain. The RARα′ was found to be a dominant represser of transcription from an RA-responsive target gene; however, expression of RARaα′ was insufficient to confer RA nonresponsiveness, suggesting that RAC65 cells carry an additional mutation(s) affecting RA-induced genes.

引用

页码：6445 / 6453

页数：9

共 72 条

[41]

Kruys V.I., Wathelet M.G., Huez G.A., Identification of a translation inhibitory element (TIE) in the 3′ untranslated region of the human interferon-beta mRNA, Gene, 72, pp. 191-200, (1988)

[42]

Langille R.M., Paulsen D.F., Solursh M., Differential effects of physiological concentrations of retinoic acid in vitro on chondrogenesis and myogenesis in chick craniofacial mesenchyme, Differentiation, 40, pp. 84-92, (1989)

[43]

LaRosa G.J., Gudas L.J., Early retinoic acid-induced F9 teratocarcinoma stem cell gene ERA-1: Alternate splicing creates transcripts for a homeobox-containing protein and one lacking the homeobox, Mol. Cell. Biol., 8, pp. 3906-3917, (1988)

[44]

Lemishka I.R., Farmer S., Rancaniello V.R., Sharp P.A., Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA, J. Mol. Biol., 150, pp. 101-120, (1981)

[45]

Littlefield J.W., Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants, Science, 145, (1964)

[46]

Lotan R., Effects of vitamin A and its analogs (retinoids) on normal and neoplastic growth, Biochim. Biophys. Acta, 605, pp. 33-91, (1980)

[47]

Maden M., Vitamin A and pattern formation in the regenerating limb, Nature (London), 295, pp. 672-675, (1982)

[48]

Mangelsdorf D.J., Ong E.S., Dyck J.A., Evans R.M., Nuclear receptor that identifies a novel retinoic acid response pathway, Nature (London), 345, pp. 224-229, (1990)

[49]

Maniatis T., Fritsch E.F., Sambrook J., Molecular Cloning: A Laboratory Manual, (1982)

[50]

Mavilio F., Simeone A., Boncinelli E., Andrews P.W., Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid, Differentiation, 37, pp. 73-79, (1988)

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