A DOMINANT NEGATIVE MUTATION OF THE ALPHA-RETINOIC ACID RECEPTOR GENE IN A RETINOIC ACID-NONRESPONSIVE EMBRYONAL CARCINOMA CELL

被引:152
作者
PRATT, MAC [1 ]
KRALOVA, J [1 ]
MCBURNEY, MW [1 ]
机构
[1] UNIV OTTAWA,DEPT MED,451 SMYTH RD,OTTAWA K1H 8M5,ONTARIO,CANADA
关键词
D O I
10.1128/MCB.10.12.6445
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pluripotential embryonal carcinoma cells such as those of the P19 line differentiate when exposed to retinoic acid (RA). The RAC65 cell line is a mutant clone of P19 cells selected to be RA nonresponsive. RAC65 cells carry a rearrangement affecting one of the genes encoding a nuclear retinoic acid receptor (RARα). The mutant gene encodes a protein, RARα′, that has lost its 70 C-terminal amino acids, thus truncating the RA-binding domain. The RARα′ was found to be a dominant represser of transcription from an RA-responsive target gene; however, expression of RARaα′ was insufficient to confer RA nonresponsiveness, suggesting that RAC65 cells carry an additional mutation(s) affecting RA-induced genes.
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收藏
页码:6445 / 6453
页数:9
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