BETA-GLOBIN DOMINANT CONTROL REGION INTERACTS DIFFERENTLY WITH DISTAL AND PROXIMAL PROMOTER ELEMENTS

被引：98

作者：

ANTONIOU, M

GROSVELD, F

机构：

来源：

GENES & DEVELOPMENT | 1990年 / 4卷 / 06期

关键词：

dominant control region; transcription; β-globin;

D O I：

10.1101/gad.4.6.1007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

We have studied the interaction between the dominant control region (DCR) and the promoter of the human β-globin gene. Expression analysis in MEL cells has revealed that the DCR contains a number of elements capable of replacing the upstream (-250 to -100) erythroid-specific region of the promoter. The DCR strongly stimulates expression from a promoter possessing only a TATA box. However, this basic level of transcription is not induced upon erythroid differentiation of the cells. Mutational analysis of the minimal (-100, noninducible) promoter shows that only the combination of the DCR and the CAC/CCAAT elements provides erythroid-specific transcription. These regions act synergistically to produce full regulated expression during erythroid differentiation.

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页码：1007 / 1013

页数：7

相关论文

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