A METHOD FOR THE STABILIZATION OF RECOMBINANT PLASMIDS IN YEAST

被引：15

作者：

CAUNT, P

IMPOOLSUP, A

GREENFIELD, PF

机构：

[1] UNIV QUEENSLAND,DEPT CHEM ENGN,ST LUCIA,QLD 4067,AUSTRALIA

[2] UNIV QUEENSLAND,CTR MOLEC BIOL & BIOTECHNOL,ST LUCIA,QLD 4067,AUSTRALIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 14卷 / 3-4期

关键词：

Dissolved oxygen tension cycling; Non-selective medium; Recombinant plasmid; Saccharomyces cerevisiae; Stability; Yeast;

D O I：

10.1016/0168-1656(90)90115-R

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The stability of a yeast plasmid can be improved using deliberately induced cyclic changes in the dissolved oxygen tension (DOT), during continuous culture in a non-selective, undefined medium. The resultant stability of the plasmid under DOT cycled conditions is strongly dependent on the growth rate of the culture, with complete stabilisation at lower growth rates. We propose a mechanism for the stabilisation and suggest that the method can be applied to other recombinant yeast strains. © 1990.

引用

页码：311 / 320

页数：10

共 16 条

[1] FED BATCH CULTURE OF SACCHAROMYCES-CEREVISIAE - PERSPECTIVE OF COMPUTER CONTROL TO ENHANCE PRODUCTIVITY IN BAKERS-YEAST CULTIVATION [J].