GENETIC FINGERPRINTING OF THEOBROMA CLONES USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS

被引：147

作者：

WILDE, J ^{[1
]}

WAUGH, R ^{[1
]}

POWELL, W ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST, DEPT CELL & MOLEC GENET, DUNDEE DD2 5DA, SCOTLAND

来源：

THEORETICAL AND APPLIED GENETICS | 1992年 / 83卷 / 6-7期

关键词：

COCOA; RAPDS; DNA; POLYMORPHISM; GENETIC DIVERSITY;

D O I：

10.1007/BF00226710

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Randomly amplified polymorphic DNA (RAPD) markers have been used to characterise cocoa clones representing the three main cultivated subpopulations: Criollo, Forastero and Trinitario. The use of single primers of arbitrary nucleotide sequence resulted in the selective amplification of DNA fragments which were unique to the individual cocoa clones studied. The use of a single primer allowed each of the clones evaluated to be unequivocally characterised. The application of RAPD markers for the evaluation of germplasm and cocoa improvement programmes are discussed.

引用

页码：871 / 877

页数：7

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