THE SMALL GENOME OF ARABIDOPSIS CONTAINS AT LEAST 6 EXPRESSED ALPHA-TUBULIN GENES

被引:176
作者
KOPCZAK, SD
HAAS, NA
HUSSEY, PJ
SILFLOW, CD
SNUSTAD, DP
机构
[1] UNIV MINNESOTA,DEPT GENET & CELL BIOL,ST PAUL,MN 55108
[2] UNIV MINNESOTA,DEPT PLANT BIOL,ST PAUL,MN 55108
[3] UNIV MINNESOTA,INST PLANT MOLEC GENET,ST PAUL,MN 55108
关键词
D O I
10.1105/tpc.4.5.539
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The goal of our investigations is to define the genetic control of microtubule-based processes in a higher plant. The available evidence suggests that we have achieved our first objective: the characterization of the complete alpha-tubulin and beta-tubulin gene families of Arabidopsis. Four additional alpha-tubulin genes (TUA2, TUA4, TUA5, and TUA6) of Arabidopsis have been cloned and sequenced to complete the analysis of the gene structure for all six alpha-tubulin genes detectable on DNA gel blots of Arabidopsis genomic DNA hybridized with alpha-tubulin coding sequences. TUA1 and TUA3 were characterized earlier in our laboratory. Noncoding gene-specific hybridization probes have been constructed for all six alpha-tubulin genes and used in RNA gel blot analyses to demonstrate that all six genes are transcribed. The six genes encode four different alpha-tubulin isoforms; TUA2 and TUA4 encode a single isoform, as do TUA3 and TUA5. Two-dimensional protein gel immunoblot analyses have resolved at least four alpha-tubulin isoforms from plant tissues, suggesting that all of the predicted TUA gene products are synthesized in vivo.
引用
收藏
页码:539 / 547
页数:9
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