IN-VIVO INJECTION OF ANTIBODIES DIRECTED AGAINST THE CLONED MU-OPIOID RECEPTOR BLOCKED SUPRASPINAL ANALGESIA INDUCED BY MU-AGONISTS IN MICE

The intracerebroventricular (i.c.v.) injection to mice of a polyclonal antibody raised against the peptide sequence 208-216 (TKYRQGSID) of cloned rat mu opioid receptor, reduced the analgesic potency of DAMGO, morphine and beta-endorphin-(1-31) when studied 48 h later in the tail-flick test. Antinociception elicited by delta agonists, DPDPE and [D-Ala(2)]-Deltorphin II, and by the kappa agonist U-50488H, was fully expressed in mice undergoing this treatment. The specific binding displayed by 0.6 nM [H-3]-DAMGO was reduced in membranes preincubated with the antiserum, whereas no change could be detected for 3 nM [H-3]-DPDPE or 2 nM [H-3]-U-69593 labelling delta and kappa opioid receptors respectively. Naloxonazine, irreversible antagonist of the pharmacologically defined mu(1) opioid receptor, and beta-funaltrexamine (beta-FNA), that also displays irreversible antagonism at mu(1/2) receptors, when injected i.c.v. 24 h before the opioids significantly reduced the activity of DAMGO and morphine, In mice treated with naloxonazine, but not with beta-FNA, the antibody further reduced the remaining analgesic effect of DAMGO and morphine. Thus, both the antibody and beta-FNA blocked a wider population of mu opioid receptors than that tagged by naloxonazine.