THEORETICAL AND EXPERIMENTAL EPITOPE MAPPING OF THYMOSIN BETA(4)

被引：7

作者：

BECKER, S ^{[1
]}

ARMBRUSTER, FP ^{[1
]}

MULLER, B ^{[1
]}

ECHNER, H ^{[1
]}

KAPURNOTU, A ^{[1
]}

LIVANIOU, E ^{[1
]}

MIHELIC, M ^{[1
]}

STOEVA, S ^{[1
]}

VOELTER, W ^{[1
]}

机构：

[1] IMMUNDIAGNOST GMBH,D-64625 BENSHEIM,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 177卷 / 1-2期

关键词：

THYMOSIN BETA(4); EPITOPE MAPPING; ANTIGENIC DETERMINANT; THYMUS PEPTIDE;

D O I：

10.1016/0022-1759(94)90150-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two rabbit polyclonal antisera, one directed against thymosin beta(4) and the other one against the peptide fragment thymosin beta(4) (1-14) were characterised by epitope mapping. Hexapeptides representing the whole sequence of the native peptide and overlapping by one amino acid were synthesised on polystyrene pins. The antigenic determinants were identified in microtitre plates with an ELISA procedure. The polyclonal serum against thymosin beta(4) detected three epitopes (N-terminal, mid-region and C-terminal) whereas the polyclonal serum against the fragment contained only antibodies specific for the N-terminal epitope. These experimental results are consistent with theoretical predictions.

引用

页码：131 / 137

页数：7

共 17 条

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