CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF DOUBLE-HELICAL RNA OCTAMERS

被引：7

作者：

EGLI, M ^{[1
]}

PORTMANN, S ^{[1
]}

TRACZ, D ^{[1
]}

WORKMAN, C ^{[1
]}

USMAN, N ^{[1
]}

机构：

[1] RIBOZYME PHARMACEUT INC, BOULDER, CO 80301 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1995年 / 51卷

关键词：

D O I：

10.1107/S0907444995003817

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals of a chemically synthesized self-complementary RNA octamer with sequence r(CCCCGGGG) have been obtained by screening 50 different conditions at room temperature using a standard sparse-matrix sampling method. Two crystal forms with different morphologies grew under diverse crystallization conditions within days by hanging-drop vapor diffusion. Hexagonal crystals with space group P6(1)22 (one strand per asymmetric unit) and unit-cell dimensions a = b = 39.73 and c = 58.55 Angstrom diffracted to 2.6 Angstrom. Rhombohedral crystals with space group R32 (one duplex per asymmetric unit) and unit-cell dimensions a=b=42.38 and c=131.70 Angstrom, (hexagonal setting) diffracted beyond 1.5 Angstrom. Data sets for both crystal forms were collected on image-plate/rotating-anode generator equipment and structure determinations and refinements are under way.

引用

页码：1065 / 1070

页数：6

共 38 条

[1] STRUCTURE OF AN RNA DOUBLE HELIX INCLUDING URACIL-URACIL BASE-PAIRS IN AN INTERNAL LOOP [J].