DETECTION OF EPERYTHROZOON-SUIS DNA FROM SWINE BLOOD BY WHOLE ORGANISM DNA HYBRIDIZATIONS

被引：4

作者：

OBERST, RD ^{[1
]}

HALL, SM ^{[1
]}

SCHONEWEIS, DA ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI, COLL VET MED, DEPT SURG & MED, MANHATTAN, KS 66506 USA

来源：

VETERINARY MICROBIOLOGY | 1990年 / 24卷 / 02期

关键词：

D O I：

10.1016/0378-1135(90)90060-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A procedure is described for isolating Eperyhrozoon suis DNA of sufficient quantity and purity to serve as a probe in whole-organism DNA hybridizations for detecting parasitized wine. The E. suis organisms were isolated from the blood of infected swine; the DNA was recovered and digested with restriction endonucleases and resolved on agarose gels. In DNA hybridizations using recovered E. suis DNA, blood samples from parasitized swine could be differentiated from uninfected, control samples. A high salt lysate recovery technique was used in sampling swine whole blood for E. suis DNA and found to offer many advantages in the collection and recovery process. © 1990.

引用

页码：127 / 134

页数：8

共 16 条

[11]

SMITH AR, 1978, PURDUE VET NOTES, V106, P5

[12]

SPLITTER EJ, 1950, AM J VET RES, V11, P324

[13] ERYTHROCYTE-MEMBRANE ALTERATIONS ASSOCIATED WITH THE ATTACHMENT AND REPLICATION OF EPERYTHROZOON-SUIS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY [J].

ZACHARY, JF ;

BASGALL, EJ .

VETERINARY PATHOLOGY, 1985, 22 (02) :164-170

[14]

ZACHARY JF, 1985, AM J VET RES, V46, P821

[15]

ZINN GM, 1983, J AM VET MED ASSOC, V182, P369

[16] HIGH SALT LYSATES - A SIMPLE METHOD TO STORE BLOOD-SAMPLES WITHOUT REFRIGERATION FOR SUBSEQUENT USE WITH DNA PROBES [J].

ZOLG, JW ;

SCOTT, ED ;

WENDLINGER, M .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1988, 39 (01) :33-40

← 1 2 →