RAPID ISOLATION OF CARCINOGEN-BOUND DNA AND RNA BY HYDROXYAPATITE CHROMATOGRAPHY

被引：192

作者：

BELAND, FA

DOOLEY, KL

CASCIANO, DA

机构：

[1] Department of Health, Education and Welfare, Food and Drug Administration, National Center for Toxicologial Research, Jefferson

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 174卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)87048-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Carcinogen-bound DNA and RNA are conveniently isolated by solvent extraction and hydroxyapatite (HAP) chromatography. Tissue is suspended in 8 M urea-0.24 M sodium phosphate-1 % sodium dodecyl sulfate-10 mM EDTA, pH 6.8 (MUP-SDS-EDTA_ and extracted with chloroform-isoamylalcohol-phenol (24:1:25; CIP) to remove protein. RNA and DNA are separated by passing the aqueous solution through an HAP column; RNA is eluted with MUP, DNA with 0.48 M sodium phosphate, pH 6.8. Examples presented are: (1) calf thymus DNA that has been reacted with N-acetoxy-2-acetylaminofluorene (N-OAc-AAF), (2) isolated intact rat hepatocytes incubated with N-hydroxy-AAF and (3) livers from Sprague-Dawley rats treated with N-hydroxy-AAF. © 1979.

引用

页码：177 / 186

页数：10

共 45 条

[1] COMPUTER-GENERATED GRAPHIC MODELS OF N-2-SUBSTITUTED DEOXYGUANOSINE ADDUCTS OF 2-ACETYLAMINOFLUORENE AND BENZO[A]PYRENE AND O-6-SUBSTITUTED DEOXYGUANOSINE ADDUCT OF 1-NAPHTHYLAMINE IN DNA DOUBLE HELIX [J].

BELAND, FA .

CHEMICO-BIOLOGICAL INTERACTIONS, 1978, 22 (2-3) :329-339

[2]

BONNEY RJ, 1974, IN VITRO CELL DEV B, V10, P130

[3]

BRITTEN RJ, 1970, CARNEGIE I WASH YEAR, V68, P400

[4] MUTAGENICITY OF POLYCYCLIC AROMATIC-HYDROCARBONS [J].

BROOKES, P .

MUTATION RESEARCH, 1977, 39 (3-4) :257-284

[5]

CASCIANO DA, 1978, CANCER LETT, V5, P173, DOI 10.1016/S0304-3835(78)80035-4

[6] STRUCTURAL IDENTIFICATION OF MAJOR DNA ADDUCT FORMED BY AFLATOXIN-B1 INVITRO [J].

ESSIGMANN, JM ;

CROY, RG ;

NADZAN, AM ;

BUSBY, WF ;

REINHOLD, VN ;

BUCHI, G ;

WOGAN, GN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (05) :1870-1874

[7] CHANGES OF STABILITY AND CONFORMATION OF DNA FOLLOWING COVALENT BINDING OF A CARCINOGEN [J].

FUCHS, R ;

DAUNE, M .

FEBS LETTERS, 1971, 14 (04) :206-&

[8] PHYSICAL BASIS OF CHEMICAL CARCINOGENESIS BY N-2-FLUORENYLACETAMIDE DERIVATIVES AND ANALOGS [J].

FUCHS, R ;

DAUNE, M .

FEBS LETTERS, 1973, 34 (02) :295-298

[9] PHYSICAL STUDIES ON DEOXYRIBONUCLEIC ACID AFTER COVALENT BINDING OF A CARCINOGEN [J].

FUCHS, R ;

DAUNE, M .

BIOCHEMISTRY, 1972, 11 (14) :2659-&

[10] COMPARATIVE ORIENTATION OF FLUORENE RESIDUE IN NATIVE DNA MODIFIED BY N-ACETOXY-N-2-ACETYLAMINOFLUORENE AND 2 7-HALOGEN-O DERIVATIVES [J].

FUCHS, RPP ;

LEFEVRE, JF ;

POUYET, J ;

DAUNE, MP .

BIOCHEMISTRY, 1976, 15 (15) :3347-3351

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