RAPID ISOLATION OF CARCINOGEN-BOUND DNA AND RNA BY HYDROXYAPATITE CHROMATOGRAPHY

被引：192

作者：

BELAND, FA

DOOLEY, KL

CASCIANO, DA

机构：

[1] Department of Health, Education and Welfare, Food and Drug Administration, National Center for Toxicologial Research, Jefferson

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 174卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)87048-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Carcinogen-bound DNA and RNA are conveniently isolated by solvent extraction and hydroxyapatite (HAP) chromatography. Tissue is suspended in 8 M urea-0.24 M sodium phosphate-1 % sodium dodecyl sulfate-10 mM EDTA, pH 6.8 (MUP-SDS-EDTA_ and extracted with chloroform-isoamylalcohol-phenol (24:1:25; CIP) to remove protein. RNA and DNA are separated by passing the aqueous solution through an HAP column; RNA is eluted with MUP, DNA with 0.48 M sodium phosphate, pH 6.8. Examples presented are: (1) calf thymus DNA that has been reacted with N-acetoxy-2-acetylaminofluorene (N-OAc-AAF), (2) isolated intact rat hepatocytes incubated with N-hydroxy-AAF and (3) livers from Sprague-Dawley rats treated with N-hydroxy-AAF. © 1979.

引用

页码：177 / 186

页数：10

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