HUMAN AGING IS ASSOCIATED WITH VARIOUS POINT MUTATIONS IN TRANSFER-RNA GENES OF MITOCHONDRIAL-DNA

被引：86

作者：

MUNSCHER, C

MULLERHOCKER, J

KADENBACH, B

机构：

[1] UNIV MARBURG,FACHBEREICH CHEM,D-35032 MARBURG,GERMANY

[2] UNIV MUNICH,INST PATHOL,D-80337 MUNSTER,GERMANY

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1993年 / 374卷 / 12期

关键词：

AGING; POINT MUTATIONS; TRANSFER-RNA GENES; MITOCHONDRIAL DNA; POLYMERASE CHAIN REACTION;

D O I：

10.1515/bchm3.1993.374.7-12.1099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In extraocular muscle tissue of elderly humans small amounts of point mutations in tRNA genes of mitochondrial DNA (mtDNA) were identified by point mutation-specific PCR. These mutations were not found in navel-string samples from newborns. While the mutations in tRNA(Leu(UUR)) (np 3243) and tRNA(Gly) (np 10006), previously identified in patients with MELAS and CIPO, respectively, were found in most elderly people, the mutations in tRNA(Ser(GCU)) (np 12246) and tRNA(Asn) (np 5692), identified in patients with CIPO and CPEO, respectively, were found only in two of 15 tissue samples from different individuals. The data suggest that some nucleotides of mtDNA represent ''hot spots'' for somatic mutations, which contribute to human aging.

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页码：1099 / 1104

页数：6

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