Large-Scale Mapping Using High-Molecular-Weight DNA from P-vulgaris

被引：6

作者：

Creusot, Francine ^{[1
]}

Sevignac, Mireille ^{[1
]}

Dron, Michel ^{[1
]}

机构：

[1] Univ Paris 11, URA CNRS 1128, Dept Biol Mol Vegetale, F-91405 Orsay, France

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 02期

关键词：

P; vulgaris; megabase; mapping;

D O I：

10.1007/BF02668348

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of megabase methods is essential for positional cloning of genes for which no products have been characterized. We have set about the isolation of genes conferring resistance to pathogens in the french bean Phaseolus vulgaris. We describe here the isolation of high-molecular-weight DNA, pulse-field gel electrophoresis and large-scale mapping of the Pal2 locus in P. vulgaris. We describe the construction of such a map by embedding DNA isolated from leaf nuclei in agarose piugs, restricting it with rare cutters and subjecting the DNA to pulse-field gel electrophoresis.

引用

页码：207 / 218

页数：12

共 22 条

[1] AN ELECTROPHORETIC KARYOTYPE FOR YEAST [J].

CARLE, GF ;

OLSON, MV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (11) :3756-3760

[2] THE ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM WHEAT, BARLEY AND RYE FOR ANALYSIS BY PULSE-FIELD GEL-ELECTROPHORESIS [J].

CHEUNG, WY ;

GALE, MD .

PLANT MOLECULAR BIOLOGY, 1990, 14 (05) :881-888

[3] TRANSGENIC PLANTS OF LETTUCE (LACTUCA-SATIVA) OBTAINED THROUGH ELECTROPORATION OF PROTOPLASTS [J].

CHUPEAU, MC ;

BELLINI, C ;

GUERCHE, P ;

MAISONNEUVE, B ;

VASTRA, G ;

CHUPEAU, Y .

BIO-TECHNOLOGY, 1989, 7 (05) :503-&

[4] PHENYLALANINE AMMONIA-LYASE GENE ORGANIZATION AND STRUCTURE [J].

CRAMER, CL ;

EDWARDS, K ;

DRON, M ;

LIANG, XW ;

DILDINE, SL ;

BOLWELL, GP ;

DIXON, RA ;

LAMB, CJ ;

SCHUCH, W .

PLANT MOLECULAR BIOLOGY, 1989, 12 (04) :367-383

[5]

ELLIS THN, 1990, PLANT MOL BIOL, V14, P73, DOI 10.1007/BF00015656

[6] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[7] PULSED FIELD GEL-ELECTROPHORESIS AND PHYSICAL MAPPING OF LARGE DNA FRAGMENTS IN THE TM-2A REGION OF CHROMOSOME-9 IN TOMATO [J].

GANAL, MW ;

YOUNG, ND ;

TANKSLEY, SD .

MOLECULAR AND GENERAL GENETICS, 1989, 215 (03) :395-400

[8] GENETIC-LINKAGE BETWEEN MEX2, A SPECIFIC RESISTANCE GENE TO ANTHRACNOSE, AND ANP, A GENE INVOLVED IN POD ANTHOCYANIN ACCUMULATION IN BEAN [J].

GANTET, P ;

BETTINI, P ;

GRISVARD, J ;

DRON, M .

PLANT DISEASE, 1991, 75 (09) :941-942

[9] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY OF ARABIDOPSIS WHICH IS SUITABLE FOR CHROMOSOME WALKING [J].

GRILL, E ;

SOMERVILLE, C .

MOLECULAR & GENERAL GENETICS, 1991, 226 (03) :484-490

[10] SEQUENCE SPECIFICITY OF METHYLATION IN HIGHER-PLANT DNA [J].

GRUENBAUM, Y ;

NAVEHMANY, T ;

CEDAR, H ;

RAZIN, A .

NATURE, 1981, 292 (5826) :860-862

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