RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS DETECTED IN A SEGREGATING HYBRID POPULATION OF SOLANUM-CHACOENSE X SOLANUM-PHUREJA

被引:16
作者
HOSAKA, K [1 ]
HANNEMAN, RE [1 ]
机构
[1] UNIV WISCONSIN,DEPT HORT,USDA ARS,MADISON,WI 53706
来源
JAPANESE JOURNAL OF GENETICS | 1994年 / 69卷 / 01期
关键词
D O I
10.1266/jjg.69.53
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Reproducible random amplified polymorphic DNA (RAPD) patterns were obtained in two potato species (Solanum chacoense and S. phureja), one of their F1's, and its derived F2 Population, by adjusting temperature profiles of the amplification process and template DNA concentrations (1 ng/mul reaction vol.). Although the number of amplified products and detectable differences between the two species increased with increasing GC content of the primer, 50% or 60% GC is recommended for maximizing scorable RAPDs in the F2 population. Using 82 primers, 589 RAPDs were detected between the parents, and 70% of them (409 RAPDs) in the F1 clone. The number of RAPDs reliably scored for their segregations in the F2 population was significantly lowered because of complicated RAPD patterns. Consequently, 22% of the RAPDs detected in the parents (129 RAPDs), an average of 1.57 RAPDs per primer, were obtained as genetic markers, which can be used for the construction of a genetic map for this particular population.
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页码:53 / 66
页数:14
相关论文
共 34 条
[11]   USE OF RANDOM AMPLIFIED POLYMORPHIC DNAS (RAPDS) FOR IDENTIFICATION OF RICE ACCESSIONS [J].
FUKUOKA, S ;
HOSAKA, K ;
KAMIJIMA, O .
JAPANESE JOURNAL OF GENETICS, 1992, 67 (03) :243-252
[12]   RFLP MAPS OF POTATO AND THEIR ALIGNMENT WITH THE HOMOEOLOGOUS TOMATO GENOME [J].
GEBHARDT, C ;
RITTER, E ;
BARONE, A ;
DEBENER, T ;
WALKEMEIER, B ;
SCHACHTSCHABEL, U ;
KAUFMANN, H ;
THOMPSON, RD ;
BONIERBALE, MW ;
GANAL, MW ;
TANKSLEY, SD ;
SALAMINI, F .
THEORETICAL AND APPLIED GENETICS, 1991, 83 (01) :49-57
[13]   IDENTIFICATION OF 2N BREEDING LINES AND 4N VARIETIES OF POTATO (SOLANUM-TUBEROSUM, SSP TUBEROSUM) WITH RFLP-FINGERPRINTS [J].
GEBHARDT, C ;
BLOMENDAHL, C ;
SCHACHTSCHABEL, U ;
DEBENER, T ;
SALAMINI, F ;
RITTER, E .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (01) :16-22
[14]   RFLP ANALYSIS AND LINKAGE MAPPING IN SOLANUM-TUBEROSUM [J].
GEBHARDT, C ;
RITTER, E ;
DEBENER, T ;
SCHACHTSCHABEL, U ;
WALKEMEIER, B ;
UHRIG, H ;
SALAMINI, F .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (01) :65-75
[15]  
HANNEMAN RE, 1985, AM POTATO J, V62, P428
[16]  
Hawkes J.G., 1990, POTATO EVOLUTION
[17]   SEED PROTEIN VARIATION WITHIN ACCESSIONS OF WILD AND CULTIVATED POTATO SPECIES AND INBRED SOLANUM-CHACOENSE [J].
HOSAKA, K ;
HANNEMAN, RE .
POTATO RESEARCH, 1991, 34 (04) :419-428
[18]  
HU J, 1991, PLANT CELL REP, V10, P505, DOI 10.1007/BF00234583
[19]  
Kaga Akito, 1993, Science Reports of Faculty of Agriculture Kobe University, V20, P171
[20]   ISOLATION OF MOLECULAR MARKERS FOR TOMATO (LYCOPERSICON-ESCULENTUM) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) [J].
KLEINLANKHORST, RM ;
VERMUNT, A ;
WEIDE, R ;
LIHARSKA, T ;
ZABEL, P .
THEORETICAL AND APPLIED GENETICS, 1991, 83 (01) :108-114