THE CD5(+) B-CELLS AND MYASTHENIA-GRAVIS

被引：6

作者：

ARAGA, S

KISHIMOTO, M

ADACHI, A

NAKAYASU, H

TAKENAKA, T

TAKAHASHI, K

机构：

[1] Division of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University

来源：

AUTOIMMUNITY | 1995年 / 20卷 / 02期

关键词：

THYMOCYTES; 2-COLOR ANALYSIS; FLOW CYTOMETRY; THYMECTOMY;

D O I：

10.3109/08916939509001937

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A high frequency of CD5(+)B lymphocytes in the peripheral blood of patients with myasthenia gravis (MG) has been reported recently. These results seem to indicate an attractive linkage between CD5(+)B lymphocytes and autoantibodies against Acetylcholine receptor in MG. We examined the frequency of CD5+B cells in 20 patients with MG and 21 normal healthy controls by two-color flow cytometry. However, there were no significant differences in the percentages of CD5(+)B lymphocytes between the two groups. We also examined the frequency of CD5(+)B lymphocytes in the resected thymus of patients. The frequency of CD5(+)B lymphocytes in the thymus was low and similar pattern to that in the peripheral blood. We checked the antibody (Ab) production against the human acetylcholine receptor in either CD5(+)B or CD5(-)B lymphoctes using B lymphoblastoid cell line generated from the lymphocytes of 11 patients with anti-AChR Abs in the sera. Abs against the AChR in the human were mostly produced by CD5(-)B, not CD5(+)B lymphocytes. The anti-AChR Abs (IgG) production of CD5(+)B cells and CD5(-)B cells (mean +/- SD) were 6.8 +/- 2.4 fmol/ml and 18.5 +/- 17.6 fmol/ml, respectively. These results sugget that in MG, the frequencies of the CD5(+)B lymphocytes in PBL may be genetic background and that there may be no strong linages between AChR Ab production and CD5(+)B lymphocytes.

引用

页码：129 / 134

页数：6

共 26 条

[11]

Boyum A., Isolation of mononuclear cells and granulocytes from human blood, Scand. J. Clin. Lab. Invset., 21, pp. 77-89, (1977)

[12]

Frochner S.C., Rafto S., Comparison of the subunits of Torpedo californica acetylcholine receptor by peptide mapping, Biochemistry, 18, pp. 301-307, (1979)

[13]

Msaon D.W., Penhale W.J., Sedgwick J.D., Preparation of lymphocyte subpopulation, Lymphocytes: a practical approach, pp. 48-52, (1987)

[14]

Walls E.V., Crawford D.H., Generation of human B lymphoblastoid cell lines using Epstein-Barr virus, Lymphocytes-a practical approach, pp. 149-162, (1987)

[15]

Casali P., Prabhakar B.S., Notkins A.L., Characterization of multireactive autoantibodies and identification of LEU-1+ B lymphocytes as cells making antibodies binding multiple self and exogenous molecules, Int. Rev. Immunol, 3, pp. 17-45, (1988)

[16]

Casali P., Notkins A.L., Probing the human B-cell repertoire with EBV: Polyrcactive antibodies and CD5+ B lymphocytes, Ann. Rev. Immunol., 7, pp. 513-535, (1989)

[17]

Kasaian M.T., Ikematsu H., Casali P., CD5+ B lymphocytes, Proc. Soc. Exp. Biol. Med., 197, pp. 226-241, (1991)

[18]

Jasin H.E., The relationship between circulating CD5+ B lymphocytes and in vitro autoantibody synthesis in normal individuals, Cell. Immunol., 136, pp. 133-141, (1991)

[19]

Lefvert A.K., Bergstrom K., Matell G., Osterman P.O., Pirskanen R., Determination of acetylcholine receptor antibody in myasthenia gravis: clinical usefulness and pathognetic implications, J. Neurol. Neurosurg. Psychiatry., 41, pp. 394-405, (1978)

[20]

Tindall R.S., Humoral immunity in myasthenia gravis: clinical correlations of anti-receptor antibody avidity and titer, Ann. NY. Acad. Sci., 377, pp. 316-331, (1981)

← 1 2 3 →