ANALYSIS OF SINGLE CELLS BY CAPILLARY ELECTROPHORESIS WITH ON COLUMN DERIVATIZATION AND LASER-INDUCED FLUORESCENCE DETECTION

被引：176

作者：

GILMAN, SD

EWING, AG

机构：

[1] 152 Davey Laboratory, Department of Chemistry, Pennsylvania State University, Pennsylvania, University Park

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 01期

关键词：

D O I：

10.1021/ac00097a010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-mu m-i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2,3-dicarboxaldehyde and CN-. Average values of compounds determined in these cells range from 180 +/- 110 amol/ cell for aspartic acid to 5.1 +/- 1.5 fmol/cell for taurine.

引用

页码：58 / 64

页数：7

共 43 条

[1] THE DIFFERENTIAL-EFFECTS OF CELL-DENSITY AND NGF ON THE EXPRESSION OF TYROSINE-HYDROXYLASE AND DOPAMINE BETA-HYDROXYLASE IN PC12 CELLS [J].