NODULE FORMATION IN SOYBEANS BY EXOPOLYSACCHARIDE MUTANTS OF RHIZOBIUM-FREDII USDA-191

被引：17

作者：

KO, YH ^{[1
]}

GAYDA, R ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,DEPT MICROBIOL,BATON ROUGE,LA 70803

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-1-105

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Production of exopolysaccharides by Rhizobium has been linked with efficient invasion and nodulation of leguminous plant roots by the bacteria. Exopolysaccharide-deficient (exo) mutants of Rhizobium fredii USDA 191 were isolated following Tn5-insertion mutagenesis. Five phenotypically unique exo mutants were investigated for exopolysaccharide synthesis and their ability to nodulate soybeans. The exopolysaccharides produced by these mutants were analysed for polysaccharide composition by column chromatography and thin-layer chromatography. Two mutants designated exo-3 and exo-5 were deficient in both neutral glucan and exopolysaccharide synthesis, but each induced some functional nodules on Glycine max (Peking). The remaining three mutants (exo-1, exo-2 and exo-4) synthesized neutral glucans at levels higher or lower than those in the wild-type and exhibited partial exopolysaccharide deficiencies. The data imply that neither exopolysaccharides nor neutral glucans are essential for the induction of determinate nodules by R. fredii.

引用

页码：105 / 113

页数：9

共 49 条

[11] NODULATION OF LEGUMES BY RHIZOBIUM - MINIREVIEW THE RECOGNIZED ROOT
DOWNIE, JA
JOHNSTON, AWB
[J]. CELL, 1986, 47 (02) : 153 - 154
[12] THE EXTRACELLULAR GLUCANS OF RHIZOBIUM-JAPONICUM STRAIN 3L1B71A
DUDMAN, WF
JONES, AJ
[J]. CARBOHYDRATE RESEARCH, 1980, 84 (02) : 358 - 364
[13] SYMBIOTIC MUTANTS OF RHIZOBIUM MELILOTI THAT UNCOUPLE PLANT FROM BACTERIAL DIFFERENTIATION
FINAN, TM
HIRSCH, AM
LEIGH, JA
JOHANSEN, E
KULDAU, GA
DEEGAN, S
WALKER, GC
SIGNER, ER
[J]. CELL, 1985, 40 (04) : 869 - 877
[14] ALTERATION OF SURFACE-PROPERTIES IN A TN5 MUTANT STRAIN OF RHIZOBIUM-TRIFOLII 0403
GARDIOL, AE
HOLLINGSWORTH, RI
DAZZO, FB
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (03) : 1161 - 1167
[15] GOODCHILD DJ, 1977, INT REV CYTOLOGY S, V6, P235
[16] SIGNAL EXCHANGE IN PLANT-MICROBE INTERACTIONS
HALVERSON, LJ
STACEY, G
[J]. MICROBIOLOGICAL REVIEWS, 1986, 50 (02) : 193 - 225
[17] MODE OF INFECTION, NODULATION SPECIFICITY, AND INDIGENOUS PLASMIDS OF 11 FAST-GROWING RHIZOBIUM-JAPONICUM STRAINS
HERON, DS
PUEPPKE, SG
[J]. JOURNAL OF BACTERIOLOGY, 1984, 160 (03) : 1061 - 1066
[18] TRANSPOSON TN5-INDUCED MUTAGENESIS OF RHIZOBIUM-JAPONICUM YIELDING A WIDE VARIETY OF MUTANTS
HOM, SSM
URATSU, SL
HOANG, F
[J]. JOURNAL OF BACTERIOLOGY, 1984, 159 (01) : 335 - 340
[19] EXTRACELLULAR POLYSACCHARIDE COMPOSITION, EX PLANTA NITROGENASE ACTIVITY, AND DNA HOMOLOGY IN RHIZOBIUM-JAPONICUM
HUBER, TA
AGARWAL, AK
KEISTER, DL
[J]. JOURNAL OF BACTERIOLOGY, 1984, 158 (03) : 1168 - 1171
[20] THE 2 MEGAPLASMIDS OF RHIZOBIUM-MELILOTI ARE INVOLVED IN THE EFFECTIVE NODULATION OF ALFALFA
HYNES, MF
SIMON, R
MULLER, P
NIEHAUS, K
LABES, M
PUHLER, A
[J]. MOLECULAR AND GENERAL GENETICS, 1986, 202 (03): : 356 - 362

← 1 2 3 4 5 →