COLUMN-SWITCHING SOLID-PHASE TRACE-ENRICHMENT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR MEASUREMENT OF BUPRENORPHINE AND NORBUPRENORPHINE IN HUMAN PLASMA AND URINE BY ELECTROCHEMICAL DETECTION

被引：30

作者：

SCHLEYER, E ^{[1
]}

LOHMANN, R ^{[1
]}

ROLF, C ^{[1
]}

GRALOW, A ^{[1
]}

KAUFMANN, CC ^{[1
]}

UNTERHALT, M ^{[1
]}

HIDDEMANN, W ^{[1
]}

机构：

[1] UNIV MUNSTER,DEPT ANESTHESIOL & INTENS CARE MED,W-4400 MUNSTER,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 614卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80319-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a new high-performance liquid chromatographic method using electrochemical detection for the determination of buprenorphine and norbuprenorphine in plasma and urine. The minimum concentration for detection of buprenorphine and norbuprenorphine is 40 pg/ml. The intra-assay coefficient of variation (C.V.) in plasma and urine samples ranges from 6 to 17% depending on the drug concentration. At a plasma concentration of 500 pg/ml the inter-assay C.V. is 8% for buprenorphine and 9% for norbuprenorphine. The analysis duration is 16 min. After solid-phase extraction and evaporation a valve-switching system with two Rheodyne valves enables sample enrichment, optimal sample cleaning and rapid elution of long-retained substances.

引用

页码：275 / 283

页数：9

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