CHARACTERIZATION OF A SAMPLING UNIT BASED ON TANGENTIAL FLOW FILTRATION FOR ONLINE BIOPROCESS MONITORING

被引：20

作者：

BUTTLER, T

GORTON, L

MARKOVARGA, G

机构：

[1] Department of Analytical Chemistry, University of Lund, S-221 00 Lund

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 279卷 / 01期

关键词：

LIQUID CHROMATOGRAPHY; PROCESS ANALYSIS; AMINO ACIDS; CARBOHYDRATES; FERMENTATION; FILTRATION; MEMBRANE FILTRATION; SAMPLING;

D O I：

10.1016/0003-2670(93)85063-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The performance of a sampling unit (Waters Filter/Acquisition Module) based on tangential flow filtration, in conjuction with column liquid chromatography, is described. Filtration characteristics of this module were investigated. Two membrane types and pore sizes and their influence on the sampling were determined. The influence of feed rate on the flow-rate of the filtrate for model solutions and real fermentation substrates and broths was investigated. Membrane recovery values for carbohydrates in solutions varying in complexity were determined. The contents of amino acids and organic acids in these substrates and broths and their recovery values are presented.

引用

页码：27 / 37

页数：11

共 18 条

[1]

BUTTLER TA, UNPUB ANAL CHEM

[2] MONITORING OF SUBSTRATES AND PRODUCTS DURING FED-BATCH PENICILLIN FERMENTATIONS ON COMPLEX MEDIA [J].