DEGRADATION OF ACETYLCHOLINE IN HUMAN AIRWAYS - ROLE OF BUTYRYLCHOLINESTERASE

被引：57

作者：

NOREL, X ^{[1
]}

ANGRISANI, M ^{[1
]}

LABAT, C ^{[1
]}

GORENNE, I ^{[1
]}

DULMET, E ^{[1
]}

ROSSI, F ^{[1
]}

BRINK, C ^{[1
]}

机构：

[1] FIRST FAC MED & SURG NAPLES,DEPT PHARMACOL & TOXICOL,I-80138 NAPLES,ITALY

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1993年 / 108卷 / 04期

关键词：

HUMAN BRONCHUS; BUTYRYLCHOLINESTERASE; ACETYLCHOLINESTERASE; ACETYLCHOLINE; BUTYRYLCHOLINE; TETRAISOPROPYLPYROPHOS-PHORAMIDE; NEOSTIGMINE;

D O I：

10.1111/j.1476-5381.1993.tb13486.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1 Neostigmine and BW284C51 induced concentration-dependent contractions in human isolated bronchial preparations whereas tetraisopropylpyrophosphoramide (iso-OMPA) was inactive on airway resting tone. 2 Neostigmine (0. 1 mum) or iso-OMPA (I 00 mum) increased acetylcholine sensitivity in human isolated bronchial preparations but did not alter methacholine or carbachol concentration-effect curves. 3 In the presence of iso-OMPA (10 mum) the bronchial rings were more sensitive to neostigmine. The pD2 values were, control: 6.05 +/- 0.15 and treated: 6.91 +/- 0.14. 4 Neostigmine or iso-OMPA retarded the degradation of acetylcholine when this substrate was exogenously added to human isolated airways. A marked reduction of acetylcholine degradation was observed in the presence of both inhibitors. Exogenous butyrylcholine degradation was prevented by iso-OMPA (10 mum) but not by neostigmine (0. 1 mum). 5 These results suggest the presence of butyrylcholinesterase activity in human bronchial muscle and this enzyme may co-regulate the degradation of acetylcholine in this tissue.

引用

页码：914 / 919

页数：6

共 26 条

[21]

MITTAG TW, 1971, ARCH INT PHARMACOD T, V191, P270

[22] CATECHOLAMINE-CONTAINING AND ACETYLCHOLINESTERASE-CONTAINING NERVES IN HUMAN LOWER RESPIRATORY-TRACT [J].

PARTANEN, M ;

LAITINEN, A ;

HERVONEN, A ;

TOIVANEN, M ;

LAITINEN, LA .

HISTOCHEMISTRY, 1982, 76 (02) :175-188

[23] EFFECTS OF INHALED CHOLINESTERASE-INHIBITORS ON BRONCHIAL TONUS AND ON PLASMA AND ERYTHROCYTE ACETYLCHOLINE ESTERASE-ACTIVITY IN RATS [J].

PAULUHN, J ;

MACHEMER, L ;

KIMMERLE, G .

TOXICOLOGY, 1987, 46 (02) :177-190

[24] THE EFFECTS OF EPITHELIUM REMOVAL ON THE ACTIONS OF CHOLINOMIMETIC DRUGS IN OPENED SEGMENTS AND PERFUSED TUBULAR PREPARATIONS OF GUINEA-PIG TRACHEA [J].

SMALL, RC ;

GOOD, DM ;

DIXON, JS ;

KENNEDY, I .

BRITISH JOURNAL OF PHARMACOLOGY, 1990, 100 (03) :516-522

[25] INVITRO EFFECTS OF VARIOUS CHOLINESTERASE-INHIBITORS ON ACETYLCHOLINESTERASE AND BUTYRYLCHOLINESTERASE OF HEALTHY-VOLUNTEERS [J].

THOMSEN, T ;

ZENDEH, B ;

FISCHER, JP ;

KEWITZ, H .

BIOCHEMICAL PHARMACOLOGY, 1991, 41 (01) :139-141

[26] CHANGES IN THE LEVELS AND FORMS OF RAT PLASMA CHOLINESTERASES DURING CHRONIC DIISOPROPYLPHOSPHOROFLUORIDATE INTOXICATION [J].

TRAINA, ME ;

SERPIETRI, LA .

BIOCHEMICAL PHARMACOLOGY, 1984, 33 (04) :645-653

← 1 2 3 →