IDENTIFICATION OF DENATURED DOUBLE-STRANDED DNA BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

被引：16

作者：

BENNER, WH

HORN, D

KATZ, J

JAKLEVIC, J

机构：

[1] Human Genome Center Instrumentation Group, Lawrence Berkeley Laboratory, University of California, Berkeley, California

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 06期

关键词：

D O I：

10.1002/rcm.1290090613

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We created double-stranded DNA (dsDNA) by annealing synthetic oligonucleotides and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to measure the difference in molecular weight of the complementary strands after denaturation. Refined sample preparation and deliberate rectilinear construction of a linear mass spectrometer produced a mass resolution exceeding 1000 for single-stranded DNA. We present methodologies and demonstrate the potential for analysing dsDNA by determining the mass of the complementary strands.

引用

页码：537 / 540

页数：4

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