DETECTION OF 500-NUCLEOTIDE DNA BY LASER-DESORPTION MASS-SPECTROMETRY

被引：102

作者：

TANG, K

TARANENKO, NI

ALLMAN, SL

CHANG, LY

CHEN, CH

机构：

[1] OAK RIDGE NATL LAB,DIV HLTH SCI RES,CHEM PHYS SECT,PHOTOPHYS GRP,OAK RIDGE,TN 37831

[2] VANDERBILT UNIV,NASHVILLE,TN 37240

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 09期

关键词：

D O I：

10.1002/rcm.1290080913

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.

引用

页码：727 / 730

页数：4

共 17 条

[1] MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF RESTRICTION ENZYME-DIGESTED PLASMID DNA USING AN ACTIVE NAFION SUBSTRATE
BAI, J
LIU, YH
LUBMAN, DM
SIEMIENIAK, D
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (09) : 687 - 691
[2] A NEW 5-YEAR PLAN FOR THE UNITED-STATES HUMAN GENOME PROJECT
COLLINS, F
GALAS, D
[J]. SCIENCE, 1993, 262 (5130) : 43 - 46
[3] SECONDARY-ELECTRON EMISSION INDUCED BY IMPACT OF LOW-VELOCITY MOLECULAR-IONS ON A MICROCHANNEL PLATE
GENO, PW
MACFARLANE, RD
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1989, 92 : 195 - 210
[4] MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION OF NONVOLATILE COMPOUNDS
KARAS, M
BACHMANN, D
BAHR, U
HILLENKAMP, F
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 78 : 53 - 68
[5] HIGH-SPEED AUTOMATED DNA SEQUENCING IN ULTRATHIN SLAB GELS
KOSTICHKA, AJ
MARCHBANKS, ML
BRUMLEY, RL
DROSSMAN, H
SMITH, LM
[J]. BIO-TECHNOLOGY, 1992, 10 (01): : 78 - 81
[6] LARDEGREN U, 1988, SCIENCE, V242, P229
[7] CAPILLARY ARRAY ELECTROPHORESIS - AN APPROACH TO HIGH-SPEED, HIGH-THROUGHPUT DNA SEQUENCING
MATHIES, RA
HUANG, XC
[J]. NATURE, 1992, 359 (6391) : 167 - 169
[8] MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES
PARR, GR
FITZGERALD, MC
SMITH, LM
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (06) : 369 - 372
[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
NICKLEN, S
COULSON, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5463 - 5467
[10] THE FUTURE OF DNA-SEQUENCING
SMITH, LM
[J]. SCIENCE, 1993, 262 (5133) : 530 - 532

← 1 2 →