DETECTION OF 500-NUCLEOTIDE DNA BY LASER-DESORPTION MASS-SPECTROMETRY

被引：102

作者：

TANG, K

TARANENKO, NI

ALLMAN, SL

CHANG, LY

CHEN, CH

机构：

[1] OAK RIDGE NATL LAB,DIV HLTH SCI RES,CHEM PHYS SECT,PHOTOPHYS GRP,OAK RIDGE,TN 37831

[2] VANDERBILT UNIV,NASHVILLE,TN 37240

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 09期

关键词：

D O I：

10.1002/rcm.1290080913

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.

引用

收藏

页码：727 / 730

页数：4

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