PURIFICATION OF THE CHLOROPLASTIC VALYL-TRANSFER RNA-SYNTHETASE FROM EUGLENA-GRACILIS

被引：19

作者：

IMBAULT, P

SARANTOGLOU, V

WEIL, JH

机构：

[1] Institut de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, 67084 Strasbourg, 15, rue Descartes

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91698-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Euglena gracilis chloroplast valyl-tRNA synthetase was purified 990 fold to a specific activity of about 1100 units/mg protein, by a series of steps including ammonium sulfate precipitation and chromatography on hydroxyapatite, DEAE-cellulose, Blue Dextran - Sepharose and Sephadex G200. The enzyme gives a single band upon polyacrylamide gel electrophoresis, appears to be a monomer with a molecular weight of 126,000 daltons and has Km values of 1.5 × 10-5 M for L-valine, 5 × 10-5 M for ATP, and 6 × 10-8 for tRNAVal. © 1979.

引用

页码：75 / 84

页数：10

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