2 PROTEINASE ACTIVITIES IN HCV POLYPEPTIDE EXPRESSED IN INSECT CELLS USING BACULOVIRUS VECTOR

被引：36

作者：

HIROWATARI, Y

HIJIKATA, M

TANJI, Y

NYUNOYA, H

MIZUSHIMA, H

KIMURA, K

TANAKA, T

KATO, N

SHIMOTOHNO, K

机构：

[1] NATL CANC CTR,RES INST,DIV VIROL,5-H TSUKIJI,CHUO KU,TOKYO 104,JAPAN

[2] SCI UNIV TOKYO,FAC SCI & TECHNOL,DEPT APPL BIOL SCI,TOKYO 162,JAPAN

来源：

ARCHIVES OF VIROLOGY | 1993年 / 133卷 / 3-4期

关键词：

D O I：

10.1007/BF01313774

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P1 and P1' of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

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页码：349 / 356

页数：8

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