AN ENZYMIC METHOD FOR DETERMINATION OF INORGANIC PYROPHOSPHATE AND ITS USE AS AN ASSAY FOR RNA POLYMERASE

被引:56
作者
JOHNSON, JC
SHANOFF, M
BASS, ST
BOEZI, JA
HANSEN, RG
机构
[1] Department of Biochemistry, Michigan State University, East Lansing
[2] Department of Microbiology, Michigan State University, East Lansing
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0003-2697(68)90037-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. An enzymic method for the determination of PP1 has been developed. PP1 can be quantitatively estimated from the amount of NADPH formed via the action of UDP-glucose pyrophosphorylase, phosphoglucomutase, and glucose-6-phosphate dehydrogenase. Neither P1 nor any of the organic phosphate compounds tested interfered in the PP1 determination. 2. 2. The method has been used for the assay of DNA-dependent RNA polymerase-by coupling the generation of PP1 by RNA polymerase with NADPH formation. The RNA polymerase catalyzed syntheses of RNA and polyriboadenylate have been assayed by this procedure. © 1968.
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页码:137 / &
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