AN ENZYMIC METHOD FOR DETERMINATION OF INORGANIC PYROPHOSPHATE AND ITS USE AS AN ASSAY FOR RNA POLYMERASE

被引：56

作者：

JOHNSON, JC

SHANOFF, M

BASS, ST

BOEZI, JA

HANSEN, RG

机构：

[1] Department of Biochemistry, Michigan State University, East Lansing

[2] Department of Microbiology, Michigan State University, East Lansing

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 26卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(68)90037-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. An enzymic method for the determination of PP1 has been developed. PP1 can be quantitatively estimated from the amount of NADPH formed via the action of UDP-glucose pyrophosphorylase, phosphoglucomutase, and glucose-6-phosphate dehydrogenase. Neither P1 nor any of the organic phosphate compounds tested interfered in the PP1 determination. 2. 2. The method has been used for the assay of DNA-dependent RNA polymerase-by coupling the generation of PP1 by RNA polymerase with NADPH formation. The RNA polymerase catalyzed syntheses of RNA and polyriboadenylate have been assayed by this procedure. © 1968.

引用

页码：137 / &

共 25 条

[21] SUB-CELLULAR DISTRIBUTION OF RAT-LIVER INORGANIC PYROPHOSPHATASE ACTIVITY [J].