CHARACTERIZATION OF BORRELIA-BURGDORFERI ASSOCIATED ANTIGENS BY MONOCLONAL-ANTIBODIES

被引：69

作者：

KRAMER, MD

SCHAIBLE, UE

WALLICH, R

MOTER, SE

PETZOLDT, D

SIMON, MM

机构：

[1] MAX PLANCK INST IMMUNBIOL, W-7800 FREIBURG, GERMANY

[2] GERMAN CANC RES CTR, INST RADIOL & PATHOPHYSIOL, ANGEW IMMUNOL ABT, W-6900 HEIDELBERG 1, GERMANY

来源：

IMMUNOBIOLOGY | 1990年 / 181卷 / 4-5期

关键词：

D O I：

10.1016/S0171-2985(11)80504-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this paper, we present a series of murine mAb recognizing B. burgdorferi antigens. The antibodies were characterized by immuno-blotting and immuno-fluorescence studies using isolates of B. burgdorferi from North America and Europe, respectively. Moreover, reactivity of the antibodies with recombinant B. burgdorferi flagellin and OspA was studied. The results suggest these anti-B. burgdorferi mAb as valuable tools for the serological analysis of B. burgdorferi isolates and for affinity-purification of the respective proteins. Moreover, these mAb appear suitable to classify antigenic variants of B. burgdorferi and to study the protective capacity of antibodies in a murine model for B. burgdorferi infection.

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页码：357 / 366

页数：10

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