PROCESSING OF RECOMBINATION INTERMEDIATES BY THE RECG AND RUVAB PROTEINS OF ESCHERICHIA-COLI

被引:122
作者
LLOYD, RG
SHARPLES, GJ
机构
[1] Department of Genetics, University of Nottingham, Queens Medical Centre
基金
英国惠康基金;
关键词
D O I
10.1093/nar/21.8.1719
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RuvAB, RuvC and RecG proteins of Escherichia coli process intermediates in recombination and DNA repair into mature products. RuvAB and RecG catalyse branch migration of Holliday junctions, while RuvC resolves these structures by nuclease cleavage around the point of strand exchange. The overlap between RuvAB and RecG was investigated using synthetic X- and Y-junctions. RuvAB is a complex of RuvA and RuvB, with RuvA providing the DNA binding subunit and RuvB the ATPase activity that drives branch migration. Both RuvA and RecG form defined complexes with each of the junctions. The gel mobilities of these complexes suggests that the X-junction attracts two tetramers of RuvA, but mainly monomers of RecG. Dissociation of the junction in the presence of ATP requires high levels of RuvAB. RecG is shown to have a much higher specific activity to the extent that very little of this protein would be required to match RuvAB in vivo. Both proteins also dissociate a Y-junction, which is consistent with helicase activity. However, RecG shows no ability to unwind more conventional substrates and the suggestion is made that its helicase activity is directed towards specific DNA structures such as junctions.
引用
收藏
页码:1719 / 1725
页数:7
相关论文
共 38 条
  • [1] EVIDENCE OF ABORTIVE RECOMBINATION IN RUV MUTANTS OF ESCHERICHIA-COLI-K12
    BENSON, F
    COLLIER, S
    LLOYD, RG
    [J]. MOLECULAR & GENERAL GENETICS, 1991, 225 (02): : 266 - 272
  • [2] NUCLEOTIDE SEQUENCING OF THE RUV REGION OF ESCHERICHIA-COLI K-12 REVEALS A LEXA REGULATED OPERON ENCODING 2 GENES
    BENSON, FE
    ILLING, GT
    SHARPLES, GJ
    LLOYD, RG
    [J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (04) : 1541 - 1549
  • [3] RESOLUTION OF HOLLIDAY JUNCTIONS INVITRO REQUIRES THE ESCHERICHIA-COLI RUVC GENE-PRODUCT
    CONNOLLY, B
    PARSONS, CA
    BENSON, FE
    DUNDERDALE, HJ
    SHARPLES, GJ
    LLOYD, RG
    WEST, SC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (14) : 6063 - 6067
  • [4] FORMATION AND RESOLUTION OF RECOMBINATION INTERMEDIATES BY ESCHERICHIA-COLI RECA AND RUVC PROTEINS
    DUNDERDALE, HJ
    BENSON, FE
    PARSONS, CA
    SHARPLES, GJ
    LLOYD, RG
    WEST, SC
    [J]. NATURE, 1991, 354 (6354) : 506 - 510
  • [5] 2 RELATED SUPERFAMILIES OF PUTATIVE HELICASES INVOLVED IN REPLICATION, RECOMBINATION, REPAIR AND EXPRESSION OF DNA AND RNA GENOMES
    GORBALENYA, AE
    KOONIN, EV
    DONCHENKO, AP
    BLINOV, VM
    [J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (12) : 4713 - 4730
  • [6] A NEW SUPERFAMILY OF REPLICATIVE PROTEINS
    HODGMAN, TC
    [J]. NATURE, 1988, 333 (6168) : 22 - 23
  • [7] ROLE OF RECA PROTEIN SPIRAL FILAMENTS IN GENETIC-RECOMBINATION
    HOWARDFLANDERS, P
    WEST, SC
    STASIAK, A
    [J]. NATURE, 1984, 309 (5965) : 215 - 220
  • [8] ESCHERICHIA-COLI RUVC PROTEIN IS AN ENDONUCLEASE THAT RESOLVES THE HOLLIDAY STRUCTURE
    IWASAKI, H
    TAKAHAGI, M
    SHIBA, T
    NAKATA, A
    SHINAGAWA, H
    [J]. EMBO JOURNAL, 1991, 10 (13) : 4381 - 4389
  • [9] ESCHERICHIA-COLI RUVA-PROTEIN AND RUVB-PROTEIN SPECIFICALLY INTERACT WITH HOLLIDAY JUNCTIONS AND PROMOTE BRANCH MIGRATION
    IWASAKI, H
    TAKAHAGI, M
    NAKATA, A
    SHINAGAWA, H
    [J]. GENES & DEVELOPMENT, 1992, 6 (11) : 2214 - 2220
  • [10] THE NUCLEOTIDE-SEQUENCE OF RECG, THE DISTAL SPO OPERON GENE IN ESCHERICHIA-COLI K-12
    KALMAN, M
    MURPHY, H
    CASHEL, M
    [J]. GENE, 1992, 110 (01) : 95 - 99