EVIDENCE OF ABORTIVE RECOMBINATION IN RUV MUTANTS OF ESCHERICHIA-COLI-K12
被引:63
作者:
BENSON, F
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机构:
UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLANDUNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLAND
BENSON, F
[1
]
COLLIER, S
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机构:
UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLANDUNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLAND
COLLIER, S
[1
]
LLOYD, RG
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h-index: 0
机构:
UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLANDUNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLAND
LLOYD, RG
[1
]
机构:
[1] UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT GENET,NOTTINGHAM NG7 2UH,ENGLAND
来源:
MOLECULAR & GENERAL GENETICS
|
1991年
/
225卷
/
02期
基金:
英国惠康基金;
关键词:
CONJUGATION;
PLASMID MOBILIZATION;
RECOMBINATION;
DNA REPAIR;
RUV GENES;
D O I:
10.1007/BF00269858
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Genetic recombination in Escherichia coli was investigated by measuring the effect of mutations in ruv and rec genes on F-prime transfer and mobilization of nonconjugative plasmids. Mutation of ruv was found to reduce the recovery of F-prime transconjugants in crosses with recB recC sbcA strains by about 30-fold and with recB recC sbcB sbcC strains by more than 300-fold. Conjugative plasmids lacking any significant homology with the chromosome were transferred normally to these ruv mutants. Mobilization of the plasmid cloning vectors pHSG415, pBR322, pACYC184 and pUC18 were reduced by 20- to 100-fold in crosses with ruv rec+ sbc+ strains, depending on the plasmid used. Recombinant plasmids carrying ruv+ were transferred efficiently. With both F-prime transfer and F-prime cointegrate mobilization, the effect of ruv was suppressed by inactivating recA. It is proposed that the failure to recover transconjugants in ruv recA+ strains is due to abortive recombination and that the ruv genes define activities which function late in recombination to help convert recombination intermediates into viable products.