TEST SYSTEM FOR DETERMINATION OF HIV-1 FRAMESHIFTING EFFICIENCY IN ANIMAL-CELLS

被引：20

作者：

REIL, H

HAUSER, H

机构：

[1] Genetics of Eukaryotes, GBF-Gesellschaft für Biotechnologische Forschung, Braunschweig

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1050卷 / 1-3期

关键词：

Frameshifting; HIV-1; Luciferase; Retrovirus; Ribosome;

D O I：

10.1016/0167-4781(90)90183-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a system in animal cells which allows the quantification of frameshifting determined by specific mRNA sequences. The method is based on the expression of an N-terminally extended firefly luciferase gene which requires frameshifting in order to be translated as a functional enzyme. The systems sensitivity is such that it allows the detection of even low efficiency of frameshifting. Our results show that the HIV-1 frameshift sequence including the 3′ located stem-loop structure leads to ribosomal frameshifting at a lower level than that described for in vitro systems when tested in several fibroblastoid cell lines. © 1990.

引用

页码：288 / 292

页数：5

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