KINETICS OF GAS DIFFUSION IN MAMMALIAN CELL CULTURE SYSTEMS .2. THEORY

被引：78

作者：

MCLIMANS, WF

BLUMENSON, LE

TUNNAH, KV

机构：

[1] Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1968年 / 10卷 / 06期

关键词：

D O I：

10.1002/bit.260100604

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mathematical models have been constructed which relate the depth of the culture fluid overlay to the oxygen available to mammalian cells cultured under static conditions. These models suggest that the maintenance of a given rate of oxygen utilization by some culture systems may be critically depended on this fluid depth and on the solubility and rate of diffusion of oxygen in the culture fluid. The importance of these concepts as applied to the isolation and growth of differentiated cells representative of the tissue of origin are noted. Copyright © 1968 John Wiley & Sons, Inc.

引用

页码：741 / +

页数：1

共 42 条

[31]

PHILLIPS HJ, 1956, P SOC EXP BIOL MED, V93, P573

[32]

PHILLIPS HJ, 1960, P SOC EXP BIOL MED, V103, P160, DOI 10.3181/00379727-103-25445

[33] EFFECT OF DINITROPHENOL ON BOVINE KIDNEY CULTURE CELLS INFECTED WITH FOOT-AND-MOUTH DISEASE VIRUS [J].