FC-ALPHA-R EXPRESSION ON POLYMORPHONUCLEAR LEUKOCYTE AND SUPEROXIDE GENERATION IN IGA NEPHROPATHY

Superoxide (O-2(-)) production and Fc alpha R antigen expression of circulating polymorphonuclear leukocytes (PMNL) isolated from patients with IgA nephropathy (IgAN) and non-IgA mesangial proliferative glomerulonephritis (PGN) and healthy volunteers were investigated to establish their biological importance in the immunopathogenesis of mesangial proliferative glomerulonephritis. PMNL from both patient groups showed increased O-2(-) production when stimulated with N-formyl methionyl leucyl phenylalanine (FMLP) and phorbol myristate acetate (PMA). The increased O-2(-) generation demonstrated a positive correlation with the degree or proteinuria. Aggregated IgA caused enhanced O-2(-) production only in patients with IgAN who also showed a significant correlation with proteinuria. Increased expression of Fc alpha R on circulating PMNL was observed in IgAN patients as determined by flow cytometric analysis. The amount of Fc alpha R on PMNL was positively correlated with O-2(-) generation triggered with IgA aggregates. These results suggest that (1.) circulating PMNL may potentially be participating in the pathogenesis of glomerular injury in mesangial proliferative glomerulonephritis, and (2.) IgA aggregates/immune complexes may contribute to the immunopathogenesis of IgAN through augmenting the Fccu receptor-mediated generation of superoxide anion.