THE HUMAN IMMUNOGLOBULIN-CHI LOCUS - PSEUDOGENES, UNIQUE AND REPETITIVE SEQUENCES

被引:23
作者
SCHABLE, KF [1 ]
THIEBE, R [1 ]
FLUGEL, A [1 ]
MEINDL, A [1 ]
ZACHAU, HG [1 ]
机构
[1] UNIV MUNICH,INST PHYSIOL CHEM,SCHILLERSTR 44,D-80336 MUNICH,GERMANY
来源
BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1994年 / 375卷 / 03期
关键词
ALU ELEMENTS; EVOLUTION; LINE1; STS; V-CHI PSEUDOGENES;
D O I
10.1515/bchm3.1994.375.3.189
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human chi locus contains 25 pseudogenes. After seven of them were described earlier the structures of the remaining 18 are reported now, thus completing the description of all human V(chi) genes and pseudogenes. Most of the pseudogenes carry several defects each. Alignments of the pseudogene sequences and comparison with the consensus sequences of the potentially functional V(chi) genes indicate that, on PCR amplification of genomic DNA aimed at certain genes of the latter class, also some of the pseudogenes would be coamplified. Unique sequences, which qualify as sequence tagged sites (STS), were defined across the locus. The occurrence of 15 repetitive elements of the LINE1 type in the locus is described. The 15 sequenced Alu elements were assigned to the known Alu subfamilies of different evolutionary age. One of the Alu elements was found only in one of the copies of the chi locus. It must, therefore, have been inserted after the duplication step which may have taken place about one million years ago. This element belongs to an Alu subfamily known to have been mobile until recently. Some aspects of the evolution of the V(chi) pseudogenes and orphons (i.e. V(chi) genes located outside the chi locus) are also discussed.
引用
收藏
页码:189 / 199
页数:11
相关论文
共 45 条
[31]   ORGANIZATION AND EVOLUTION OF A GENE-CLUSTER FOR HUMAN-IMMUNOGLOBULIN VARIABLE REGIONS OF THE KAPPA-TYPE [J].
PECH, M ;
JAENICHEN, HR ;
POHLENZ, HD ;
NEUMAIER, PS ;
KLOBECK, HG ;
ZACHAU, HG .
JOURNAL OF MOLECULAR BIOLOGY, 1984, 176 (02) :189-204
[32]   A LARGE SECTION OF THE GENE LOCUS ENCODING HUMAN-IMMUNOGLOBULIN VARIABLE REGIONS OF THE KAPPA-TYPE IS DUPLICATED [J].
PECH, M ;
SMOLA, H ;
POHLENZ, HD ;
STRAUBINGER, B ;
GERL, R ;
ZACHAU, HG .
JOURNAL OF MOLECULAR BIOLOGY, 1985, 183 (03) :291-299
[33]  
Pilbeam D., 1984, Scientific American, V250, P60
[34]   THE HUMAN VK LOCUS - CHARACTERIZATION OF EXTENDED IMMUNOGLOBULIN GENE REGIONS BY COSMID CLONING [J].
POHLENZ, HD ;
STRAUBINGER, B ;
THIEBE, R ;
PECH, M ;
ZIMMER, FJ ;
ZACHAU, HG .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 193 (02) :241-253
[35]   OF ORPHONS AND UHOS - DELIMITATION OF THE GERMLINE REPERTOIRE OF HUMAN-IMMUNOGLOBULIN KAPPA GENES [J].
ROSCHENTHALER, F ;
SCHABLE, KF ;
THIEBE, R ;
ZACHAU, HG .
BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1992, 373 (04) :177-186
[36]  
SCHABLE KF, 1993, BIOL CHEM H-S, V374, P1001
[37]  
SCHABLE KF, 1993, THESIS U MUNCHEN
[38]   UNIT-LENGTH LINE-1 TRANSCRIPTS IN HUMAN TERATOCARCINOMA CELLS [J].
SKOWRONSKI, J ;
FANNING, TG ;
SINGER, MF .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (04) :1385-1397
[39]   3 TRANSPOSED ELEMENTS IN THE INTRON OF A HUMAN VK IMMUNOGLOBULIN GENE [J].
STRAUBINGER, B ;
OSTERHOLZER, E ;
ZACHAU, HG .
NUCLEIC ACIDS RESEARCH, 1987, 15 (22) :9567-9575
[40]   MOLECULAR FOOTPRINTS OF HUMAN-IMMUNOGLOBULIN GENE EVOLUTION - A NEW SEQUENCE FAMILY [J].
STRAUBINGER, B ;
PECH, M ;
MUHLEBACH, K ;
JAENICHEN, HR ;
BAUER, HG ;
ZACHAU, HG .
NUCLEIC ACIDS RESEARCH, 1984, 12 (13) :5265-5275