THE INFLUENCE OF TROPHIC LEVEL AS MEASURED BY DELTA-N-15 ON MERCURY CONCENTRATIONS IN FRESH-WATER ORGANISMS

The relationship between mercury (Hg) concentrations in freshwater biota and trophic position, as defined by stable nitrogen isotope ratios (delta(15)N), was examined in 6 lakes in northwestern Ontario. The heavier isotope of nitrogen (N-15) increases an average of 3 parts per thousand (parts per thousand) from prey to predator and is used as a measure of an organism's trophic position. Dorsal muscle from lake trout, burbot, walleye, northern pike, white sucker, lake cisco, lake whitefish, and yellow perch was analyzed for Hg and delta(15)N using flameless atomic absorption and mass spectrometry respectively. Within each lake, log Hg was significantly related to delta(15)N (r(2) ranged from 0.47 to 0.91, P < 0.01). For four species, yellow perch, northern pike, lake cisco, and lake trout, log Hg was positively related to delta(15)N (P ranged from 0.37 to 0.47, P less than or equal to 0.09) across all lakes. We also used delta(15)N measurements (assuming a 3 parts per thousand shift between an organism and its diet) and the developed within-lake regression equations to calculate a prey Hg for each individual fish. These food Hg values were then used to predict predator Hg using Norstrom et al's bioenergetics model. Predicted results were strongly correlated to measured Hg concentrations (r = 0.91, P < 0.001), indicating that delta(15)N has potential to be used in modeling.