DISTRIBUTION OF FREE D-AMINO ACIDS IN THE TISSUES OF CRUSTACEANS

被引:51
作者
OKUMA, E [1 ]
FUJITA, E [1 ]
AMANO, H [1 ]
NODA, H [1 ]
ABE, H [1 ]
机构
[1] MIE UNIV,FAC BIORESOURCES,TSU,MIE 514,JAPAN
关键词
D-ALANINE; D-ASPARTIC ACID; D-AMINO ACID; CRUSTACEAN; (+)-1-(9-FLUORENYL)ETHYL CHLOROFORMATE;
D O I
10.2331/fishsci.61.157
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Free D-amino acids were determined in the tissues of crustaceans, three macrurans and four brachyurans, using reversed-phase ion-pair HPLC after derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate. Of several D-amino acids detected, D-alanine was the most abundant and widely distributed among every tissue of crustacean species determined. The content of D-alanine in the muscle of these crustaceans ranged from 3.2 to 16.8 mumol/g wet weight. The percentage of D-alanine to total alanine reached 31.7% on average. The second predominant D-amino acid was D-arginine which was less than 2 mumol/g and accounted for less than 10% of the total arginine. Acidic amino acids, D-aspartate and D-glutamate, were present only in the tissues of macrurans and the contents were less than 1 mumol/g. The percentage of D-aspartate to total aspartate, however, varied from 12 to 70%. Several other D-amino acids were also detected in small quantities and low percentages.
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页码:157 / 160
页数:4
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